How can we aggregate proteins in vivo, i.e when the yeast culture is growing?
Can you be a bit more specific about what you are aiming to do?
I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...
26 June 2024 3,735 3 View
Dear Members of ResearchGate, I hope you are doing well. I have been retrofitting using the ASCE 41 2017 standard. In it, I have two basic doubts. 1. The target displacement as per clause...
13 June 2024 2,773 0 View
Hello Everyone, I hope you are doing well. What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)? Thank...
30 May 2024 9,200 9 View
I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...
14 March 2024 8,131 0 View
Please can anyone suggest me how to write a book chapter in Elsevier?
21 February 2024 3,672 2 View
Hi Everyone, I want to know some of the best tools or platforms for planning research works (PhD or/and Master's research). Till now I have been using MS Excel to schedule my work, but it is not...
15 January 2024 6,985 3 View
We are purifying the protein from recombinant DNA
05 January 2024 1,086 2 View
Can someone please help me by providing the values of electronic properties (bandgap,electron and hole density of states, dielectric permittivity and electron and hole mobilities) of Niobium...
02 January 2024 3,962 1 View
Hi everyone. I wish you all a Merry Christmas and a Happy New Year ahead! I hope you are doing well. I have a question. In Opensees, why reinforced concrete beam-column joint is assumed as a...
25 December 2023 3,540 3 View
A micellar solution of protein (in PBS, pH 7.4) was loaded into the sample cell of the calorimeter. The ligand was prepared in 10% DMSO and was loaded in the injector-stirrer syringe. I have also...
22 August 2023 3,630 16 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...
01 August 2024 6,469 6 View