I am currently doing nanoparticle formulation using chitosan. The active ingredient for the formulation is polyphenol from the plant extract. So, how do I know and confirm what size of dialysis bag to use for in vitro release study. Thank you.
First, please do particle size analysis and SEM, then find the average nanoparticle size. Second, find the molecular weight of active ingredients. Finally, select the cutoff value of dialysis bag that should be below than active molecular size and/or weight (depends upon your objectives).
Most dialysis membrane has molecular weight cutoffs based on the size of protein molecules, with the most typical being 10,000 to 20,000 Da. Nanoparticles are probably going to be much larger than that (~5 nm) and will be retained.
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