I'm currently doing on entrapment of polyphenol in chitosan nanoparticles. I don't understand regarding the encapsulation efficiency of total polyphenol. In my study, I'm calculating total polyphenol in the formulation by using Folin Ciocalteu method. However, for total polyphenol, which sample should I measure? Is it the polyphenol without nanoparticles? Or is it the formulated sample without separating supernatant? I also want to ask if there other method for encapsulation efficiency since Folin Ciocalteu is old method. How to measure encapsulation efficiency by using UV-Vis? Do I need to read the spectra? Or do I need just read at max absorption which is 765 nm? Enlighten me please. Thank you.