3 horse breeds, 25 animals for each breed, genotyped using 29 microsatellite markers.
I have calculated the F-Statistics using F-STAT. The FST value is 0.006. How to test if this value is significantly different from zero. I came across few articles where a mention is made '.......FST significantly differed from zero' but do not specify the test used. In my case we know 0.006 is a low value indicating a very low genetic differentiation. How do we calculate this value is significantly different from zero? Is the purpose of finding difference from zero to see if the population is panmitic?
There are multiple methods for this, like permutation tests and so on... see for instance this discussion
https://www.researchgate.net/post/What_is_the_most_appropriate_way_to_assess_the_significance_of_pairwise_Fst_values
There are also mutilple software which perform those tests on microsatellites, Fstat or Genetix to name a few...
Genetix is a user-fiendly software. I strongly recommend it for this purpose...
You can try the user friendly software - Genetix . It will perfectly fit for your concern
Thanks every one for your help. I tried Genetix software but I couldn't exactly figure out which aspect of the results gives the indication of whether the Fst value is significantly different from zero. I have attached the result from it. Please help me locate it.
Here you have
Estimation de l'intervalle de confiance à 95% par 1000 BOOTSTRAPS SUR LES LOCUS
FIS (-0.00259 - 0.04000)
FIT ( 0.00352 - 0.04616)
FST ( 0.00099 - 0.01016)
RH (-0.00003 - 0.00051)
You can also eventually use Jackniffe standard errors results eventually...
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