Jigme Dorji

3 Questions 5 Answers 0 Followers

Questions related from Jigme Dorji

How do you choose a dye to label a primer for fragment analysis using a sequencer?

A primer is labelled with dye; for example FAM, VIC, NED etc for fragment analysis using a sequencer. We see that different primers are labelled with different dyes. Is there any basis to choose a...

15 May 2017 3,873 6 View

Can sequences of D-Loop mitochondrial DNA from various individuals in a species using different primers used for phylogentic studies?

There are different primers used in sequencing of D Loop region of mitochondrial DNA in horse. Will different primers not result in different sequences? and Can the sequences obtained using...

30 March 2017 6,162 6 View

How can we make deconjested rootless NJ tree and make the taxon readable in MEGA 7?

I have constructed Neighbor- Joinning tree using mtDNA D-loop sequences from 92 horses using MEGA 7. The dendrogram obtained is so conjested and the sequences unreadable. How do I make the...

01 January 1970 917 2 View