08 February 2017 1 455 Report

Are there any novel chemicals that are worth trying?

More Nick Chew's questions See All
What is the proper dosage for supplemental biotin in primary cell culture?

I am trying to overexpress a TurboID construct in mouse primary culture cells for validation experiments. Unfortunately the transfection of this construct appears to be killing my cells while...

26 February 2024 8,931 2 View

How to measure the DMA peak of Propolyene?

Hello every expert, When I did the DMA expertment for propolyene, I can't not get the normal spectrum. I intended to find the tg peak of PP, but it was not easy to have the beautiful peak in this...

22 February 2024 7,216 0 View

Repeated measures in three groups: which test to use?

Hello, I have an experiment in which three groups of 15 participants (disorder 1, disorder 2, control group) underwent an exercise test. I have 14 measurement points (each with a 5-min interval)....

13 December 2023 1,508 4 View

After qPCR analysis of gene expression, when is difference in relative fold change actually relevant?

During work for my undergrad thesis, I've examined and compared gene expression of stress-induced genes in plants challenged with a fungal infection. I have calculated the relative fold change and...

22 November 2023 5,518 3 View

Mushroom cultivation: can we reduce water needed for entire process while also providing drinking water for billions of people?

Our social enterprise's focus [0] is mushroom cultivation in developing countries where easy access to water cannot be assumed: 20%, globally, still have to walk away from their premise to get it...

31 October 2023 9,856 0 View

Is serum starvation necessary for cell culture?

If I am using growth medium containing BPE instead of FBS, is it still necessary for me to serum starve my cells for 24 hours before subjecting them to drug treatment? My purpose is to study cell...

14 October 2023 2,467 1 View

What is the best way to fix cells for non-permeabilized stains?

Hello, I need to stain some primary culture cells in permeabilized and non-permeabilized conditions to test if a protein is localizing to the exterior of the cell membrane. Our existing fixation...

20 September 2023 5,142 3 View

Why did I get a collection of 5 genes when I search for the gene data of SMN1?

This is what I got when I searched for SMN1 (survival of motor neuron 1) gene: https://www.ncbi.nlm.nih.gov/gene/6606 However, when I downloaded the sequence data, I found there are 5 gene...

14 August 2023 8,123 0 View

Does anyone have any idea on the range of concentration of Gefitinib that causes cell cytotoxicity in NHEK cells?

I'm trying to screen for a suitable range of concentration of Gefitinib for my subsequent ELISA assay, the final aim is to select a Gefitinib concentration that does not kill cells+give off...

31 July 2023 5,130 1 View

Can I expose my drug stock under UV in the biosafety cabinet?

I have prepared my drug stock with filtered-sterilized DMSO outside the biosafety cabinet, can I expose them under UV for a few minutes together with my cell culture tools before further diluting...

16 June 2023 6,805 3 View

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Adhesion strength of coating?

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Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

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How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

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Question about combustion Kinetics ?

I am currently working on modeling FCC catalyst regeneration and have come across a point of uncertainty that I hope you can assist me with. In my previous models, I have utilized lumped kinetics...

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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