What does it mean to set the measurement covariance matrix zero in Kalman Filter?

I am using a linear Kalman filter for estimating the position using multiple IMUs. My model is adding the accelerometer readings' double integration onto the previous positions. The state vector...

09 July 2020 5,730 2 View

What is the minimum amount of carotenoid for 600 MHz H and C NMR analysis in characterization?

We are trying to isolate some carotenoids from algae with semi-prep HPLC. As literature says, a few mg will be enough. Is this true?

07 July 2020 8,024 9 View

Do you know the cyberbullying dataset for use in machine learning?

Hi, I downloaded some social media messages on Twitter for cyberbullying detection. I want to detect cyberbullying based on machine learning through the data I have. Is there a dataset that you...

14 April 2020 2,068 1 View

Gas mixture (2 immiscible gas), One inlet - One outlet is that possible for comsol study ?

Hey, I am trying to solve gas mixture problem in comsol. I need to start inflow with one inlet, and I have gas mixture, two immiscible gas. And flow must be on one inlet through to one outlet. I...

12 July 2018 7,430 3 View

How can I evaluate mouse tumor volume equivalents in human?

Can someone help me about mice to human conversions in the field of chemotherapy? I observed approximately 14 cm3 prostate cancer tumors in my control group animals (mice). However I do not know...

13 March 2015 8,583 4 View

What are the important points for the detection of phospho-Akt in western blot?

23 October 2014 6,959 10 View

Could someone please tell me the names of botanical illustration books (or pdf)?

I would should be grateful if you could give me some names of books in Turkish.

25 July 2013 6,532 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View