I got to know that lipoprotein profiles (not only for cholesterol, but also for triglyceride) could be characterized by an automated gel-filtration system (Eur J Clin Invest 2006; 36 (2): 98–104). I was wondering whether this method was still widely-used? Or other modified method?
Lipoproteins can be separated by different methods, being ultracentrifugation and size exclusion chromatography the most used. In the paper you are referring to the assay of cholesterol and triglycerides serves to monitor the lipid content in the fractions.
The literature is plenty of methods and you can chose the one you are more familiar with the technique(/instrumentation involved. I' rather suggest to avoid using ultracentrifugation if you are not familiar with the technique.
you can find useful info here: J. Biol. Macromol., 13(2), 21-32 (2013).
Here you can find various advanced lipoprotein tests: Rifai N, Warnick GR, Dominiczak MH. Handbook of Lipoprotein
Testing. 2nd ed. Washington, DC: AACC Press; 2000.
Simplest method is to run a native gel and color them with sudan black B, compare other bands at similar place unstained. Elute them after extraction in acetone and methonol, or chloroform methanol solution, use dialysis for 10 hrs. You will get sizable aount of lipoproteins. You can use ultracentrifugation and size exclusion chromatography for lipoprotein separation.
Hi Yingyun, It depends on your purpose, if you want to know lipid profile or lipoprotein profile, of a person, for clinical diagnosis, you can use commercial methods in clinical laboratory methods, including: Enzymatic colorimetric method for total cholesterol, triglyceride, and precipitation or immuno-precipitation method for HDL-C and calculation formula for LDL-C (Friedwald formula). Also e;ectrophoresis and lipid staining method for lipoprotein assay.
But for Research intend. I suggest you at first ultra-centrifuge as golden standard method or Gel-filtration techniques. In summary, for clinical use, gel filtration is not widely use method but for research, yes it is.
best
mehdi
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