Does anyone knows about a really easy but still comprehensive ImageJ tutorial?

I am new to this software. I am in need of analyzing images from ocular ultrasounds (black/white) and find a way to quantify the internal reflectivities from the vitreous cavity in cases of...

16 February 2021 4,238 2 View

Does anybody know about literature on protest and post-conflict processes/societies? What would you recommend?

I am trying to start a project on the subject and your help would be appreciated

18 January 2021 2,190 6 View

Where to buy the pheromone dodecyl propionate?

Does anyone know where I can find dodecyl propionate? Dodecyl propionate is a rodent pheromone present in rat pup's preputial gland (original paper by Brouette-Lahlou et al., 1991, J Chem Ecol,...

18 June 2020 6,397 2 View

What are determinants of empowerment at community?

The socio-demographic and health impact factors

04 May 2020 9,019 3 View

How can you properly import a near field source in HFSS?

Hello, I am trying to import a near field source from INSIGHT to HFSS. I have the *.and file in the proper format according to the HFSS manual. I right click the work space>assign...

10 January 2020 2,598 1 View

Does anyone is working on body-mass relationship on aquatic insects in the tropics?

Hi! I am working with a species of a caddisfly from the Limnephilidae family. I am looking for a paper in which they had worked with the body-mass relationship with the equation M= aL(b). I need...

10 January 2020 568 2 View

How can i calculate the Leaf Area Index of sorghum with only the leaf area?

Hey guys, So I am trying to calculate the LAI of sorghum, but I only have the leaf area. The missing piece is the area of ground/m2. Additionally, I have the planting density, plant spacing,...

03 April 2019 8,973 3 View

Do you think using the FDA snapshot algorithm is the best way to calculate HIV-drug efficay?

Recently, the results at week 144 of E/C/F/TAF vs. E/C/F/TDF have been published. Authors state that the treatment with TAF is more effective. They used the FDA snapshot algorithm which...

04 January 2019 5,001 3 View

How can I calculate QALYs from MQOL score?

I am conducting a cost-utility analysis of a palliative care programe and I will be measuring quality of life using MQOL Questionnaire. How can I change these scores into utility values that I can...

01 February 2018 5,737 1 View

I'm looking for attitude scale related to energy consumption or saving, does anybody know one with good psychometric properties?

Hello, I'm looking for attitude scale related to energy consumption or saving, does anybody know one with good psychometric properties? Thanks! BTW, if you know a any scale that assess actual...

31 December 2015 4,210 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View