How do I use antibody stimulation in FTOC (fetal thymic organ culture)?

29 August 2022 0 2K Report

I am looking to stimulate an E17 FTOC with various antibodies. What is the best way to plate the desired antibodies? Should I plate directly into the culture media and let sit for the desired length of time?

Badges
Science topic

More Brianna Hilton's questions See All

What is the minimum sample size required for proper use of Hedge's g?

My students and I recently completed a pilot intervention study for anxiety in Division 1 volleyball players. We studied the players from one volleyball team over the course of a season, measuring...

15 February 2024 1,767 2 View

Is there a publicly available Combined Sewer Overflow (CSO) Database in the U.S.?

Is there a publicly accessible database that includes information such as when and where a combined sewer overflow event occurred? Is it at the federal level, a more decentralized level, or not...

07 September 2023 8,333 0 View

Cell Stimulation antibody plate coating?

Hello, I am planning to coat a plate for cell stimulation using a GL3 antibody at varying concentrations as well as an isotype control. My greatest concentration of GL3 antibody is 5ug/ml with no...

17 July 2022 975 0 View

What are the causes and Prevention methods of Diabetes mellitus?

Please give a simple brief summary

06 March 2022 1,546 16 View

How to get peak separation of cyclohexanol and cyclohexanone in GC-MS?

I would like to do product analysis of phenol hydrogenation products: cyclohexanone and cyclohexanol using GC-MS. I tried creating a calibration for a mixture of cyclohexanone and cyclohexanol...

30 November 2021 8,757 3 View

How does lentivirus production work?

By this, I mean how does the machinery know to insert your target gene (which has been cloned onto a viral plasmid) into the virus particle? Does anyone have any good resources on this topic?...

31 March 2021 7,858 2 View

Pros/cons of washing tissue in PBS prior to snap freezing?

Does anyone have protocol recommendations for freezing/storing human uterine tissues? I have seen some protocols that place tissues directly in LN2 and then transfer to -80 for long term storage....

02 March 2020 3,486 2 View

Method for thematic analysis without line-by-line coding?

Does anyone know of an approach to qualitative data analysis that would enable researchers to generate themes, but which does not necessitate line-by-line coding? I am familiar with some of the...

12 December 2019 9,224 8 View

What is the minimum number of observations required to use in a VAR model?

I am using a VAR model with 5 lags and 7 variables. I have monthly data with around 70 observations Is it suficient? What is the minimum number of observations required in this case?

01 October 2019 8,666 3 View

What are some sources for prices of centralized sewers and septic systems?

I am looking for costs of different waste handling systems in a variety of contexts, and was wondering if there are comprehensive sources that have already done this. I am primarily interested in...

16 September 2018 7,340 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View