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Thanks for suggestion, do u have any reference regarding second option
Many authors used 0.1 M solution of ammonium acatate to prepare bacteriophage sample for Transmission Electron Microscopy. Is isit works to precipate the phage particles or something else. Can we...
31 December 2018 6,134 3 View
Three Rs potential in the development and quality control of immunobiologicals.
01 February 2016 7,636 2 View
how to make phylogenetic tree for attached distance between the genes manually
04 May 2015 3,556 2 View
How can one decide on the dose of a new compound to analyse its effect in animal model, specially in mice
10 November 2014 3,363 3 View
I want to purify His-tagged proteins from them using Ni-NTA column chromatography, so that recombinant tagged proteins remain in their active confirmations.
09 October 2014 3,919 5 View
a case 54 year female reported of passing worms via vagina after a trip from goa,India. Microscopic view at 10X is attached here. please help to identify the the worm ..
07 August 2014 5,107 0 View
do any on have tortora and funke, text book of microiology
06 July 2014 4,078 0 View
Is there any bioinformatics method to determine acylation of protein?
03 April 2013 9,008 1 View
I am getting same intensity band in negative control of PCR reaction. How can I solve the problem without replacing the primers?
06 July 2012 6,278 20 View
I have to run a 2D sample after pull down assay using biotin labelled DNA and streptavidin beads (to isolate regulatory proteins of a DNA fragment), which buffer I should I use in pull down that...
05 June 2012 4,926 8 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Purification of gst tagged protein
25 July 2024 1,720 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View