What is the role of Ammonium Acetate in Preparation of sample of bacteriophage for transmission electron microscopy?

Many authors used 0.1 M solution of ammonium acatate to prepare bacteriophage sample for Transmission Electron Microscopy. Is isit works to precipate the phage particles or something else. Can we...

31 December 2018 5,952 3 View

Do someone has the article?

Three Rs potential in the development and quality control of immunobiologicals.

01 February 2016 7,523 2 View

How to make phylogenetic tree of attached pic data?

how to make phylogenetic tree for attached distance between the genes manually

04 May 2015 3,437 2 View

How should I decide on the drug dose for animal model (mice)?

10 November 2014 3,223 3 View

Which buffer composition should I use to lyse E. coli cells?

09 October 2014 3,790 5 View

Microbiology text book

do any on have tortora and funke, text book of microiology

06 July 2014 3,946 0 View

How do I test whether my purified protein is acetylated or not?

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31 December 2013 4,527 1 View

Posttranslational modification of protein.

Is there any bioinformatics method to determine acylation of protein?

03 April 2013 8,893 1 View

How can I get rid of a band in negative control of a PCR reaction?

I am getting same intensity band in negative control of PCR reaction. How can I solve the problem without replacing the primers?

06 July 2012 6,145 20 View

Pull down and 2D gel electrophoresis

I have to run a 2D sample after pull down assay using biotin labelled DNA and streptavidin beads (to isolate regulatory proteins of a DNA fragment), which buffer I should I use in pull down that...

05 June 2012 4,807 8 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

Access to supercomputer resources in India?

Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...

15 February 2021 8,264 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View