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What is the role of Ammonium Acetate in Preparation of sample of bacteriophage for transmission electron microscopy?

Many authors used 0.1 M solution of ammonium acatate to prepare bacteriophage sample for Transmission Electron Microscopy. Is isit works to precipate the phage particles or something else. Can we...

31 December 2018 6,134 3 View

How to make phylogenetic tree of attached pic data?

how to make phylogenetic tree for attached distance between the genes manually

04 May 2015 3,556 2 View

How should I decide on the drug dose for animal model (mice)?

How can one decide on the dose of a new compound to analyse its effect in animal model, specially in mice

10 November 2014 3,363 3 View

Which buffer composition should I use to lyse E. coli cells?

I want to purify His-tagged proteins from them using Ni-NTA column chromatography, so that recombinant tagged proteins remain in their active confirmations.

09 October 2014 3,919 5 View

Worm from vagina

a case 54 year female reported of passing worms via vagina after a trip from goa,India. Microscopic view at 10X is attached here. please help to identify the the worm ..

07 August 2014 5,107 0 View

Microbiology text book

do any on have tortora and funke, text book of microiology

06 July 2014 4,078 0 View

How do I test whether my purified protein is acetylated or not?

31 December 2013 4,645 1 View

Posttranslational modification of protein.

Is there any bioinformatics method to determine acylation of protein?

03 April 2013 9,008 1 View

How can I get rid of a band in negative control of a PCR reaction?

I am getting same intensity band in negative control of PCR reaction. How can I solve the problem without replacing the primers?

06 July 2012 6,278 20 View

Pull down and 2D gel electrophoresis

I have to run a 2D sample after pull down assay using biotin labelled DNA and streptavidin beads (to isolate regulatory proteins of a DNA fragment), which buffer I should I use in pull down that...

05 June 2012 4,926 8 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Are there any good simple systems or platforms to recommend?

In order to show people the beauty of control and enhance enthusiasm for learning control theories, are there any good simple systems or platforms to recommend?

05 August 2024 10,034 1 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View