For production of bacterial biosurfactants, it is recommended to add sterile oil to the culture broth (MSM or LB). While the medium can simply be autoclaved, how can the oil be sterilised prior to adding into the broth? Is filter sterilisation the way to go? Or is UV-sterilisation enough?
Saeed Ayaz Khan
Hi
If media can be autoclaved the oil can also be autoclaved, if not degradable, otherwise go for filter sterilization.
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Joginder Singh Duhan
Go for filter sterilization and add in the medium just before pouring, mix it well and than add in the plates.
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