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I am attempting to construct a deletion cassette of 1.497 kbp using three individual amplicons of sizes 279 bp, 1035 bp, and 243 bp (in that order). Fragment 1 (279 bp) and fragment 2 (1035 bp)...
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For production of bacterial biosurfactants, it is recommended to add sterile oil to the culture broth (MSM or LB). While the medium can simply be autoclaved, how can the oil be sterilised prior to...
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