I have found several stains for developing granule cells, but no distinct markers for mature ones. I could just use NeuN and use location, but would prefer a specific marker. Thanks
As far as I remember from my previous lab, there is not other marker for mature granular neurons than NeuN... Sorry! Why do you want to mark them? Cell sorting or immunohistochemistry? In the latter case, NeuN and localization will be enough to convince any cerebellum specialist that your cells indeed are granular neurons. Good luck!
Pax6 expression is detected in immature granular neurones of the external granular layer, migrating granular neurons ans recently settled ones in the IGL, but its expression is extinguished after P21 in wild-type mice. I don't know with which species and at what age / stage you are working, Dana, maybe can you provide us such information?
well there you go, thanks Fabrice, I've stained P21 mice with PAX6 which works well. I didn't realise it was down-regulated after.
In my opinion, the markers presented by Hussein are not specific of cerebellar granular cells: Nestin is expressed mainly (in the CNS) by neuronal / glial precursors and not mature cells, Neurofilament and MAP2 are expressed by many (most?) neurons, FOX-3 is an old alias name for NeuN, and BMPs and FGFs are so numerous that it would be useful to point a specific member of this huge families that is specific of GC. Maybe could you provide us with some references, showing these proteins to be markers of cerebellar mature GC? All my best.
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