4 Questions 8 Answers 0 Followers
Questions related from Dana Bakalar
We used TRIzol to extract RNA and proteins from some tissue (that we no longer have any left of), and in trying to run some westerns found that TRIzol is terrible for westerns. Our western...
01 January 2018 9,311 3 View
Specifically looking to replace the clickit kit with homemade buffers- almost out of buffer 1 but still have plenty of the rest for now. Does anybody use a homemade click system and willing to...
01 January 2018 7,218 4 View
I have found several stains for developing granule cells, but no distinct markers for mature ones. I could just use NeuN and use location, but would prefer a specific marker. Thanks
09 September 2016 8,865 6 View
I am trying to count cells that colocalize for two stains in a z-stack. I have got cellprofiler loading the z stack and counting, but I think its just counting all the 2d images separately and...
06 June 2016 8,046 5 View
