How do I set the energy threshold when screening RNA-RNA hybridization results?

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Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Mass spectra averaging algorithm?

I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...

22 July 2024 3,975 1 View

Concentration of gelatin as brain model ?

I am using gelatin to model mechanical properties of brain tissues in-vitro. Do you have any suggested concentration ? Thank you so much.

19 July 2024 3,095 0 View

How to obtain authoritative data on the international shipping industry?

It is best to have the route distance between ports, the size of the shipping alliance market, direct service, the number of ships owned by the shipping alliance and the congestion of ships in the...

06 July 2024 845 1 View

What are the parameters for simulating plastic deformation cracks in concrete using the CDP model?

Dear professors and colleagues， hello！Recently, while studying the CDP model, I have read the manuscripts of various experts and have gained a lot, but I still have some questions.What I...

10 June 2024 1,906 1 View

Low-yield of Cas9 plasmid after maxi-preparation. Anyone has the same issues?

Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...

22 May 2024 7,336 0 View

Your question needs a question mark?

When I calculated Kd, I found that although the concentrations were set to satisfy the type of binding used to determine the differential spectrum, Kd was unstable if the two high concentrations...

14 May 2024 399 0 View

What are the detailed mechanical characteristics of concrete?

Kindly greetings, In a conference I attended before, the peer reviewers believed that the flexural strength, compressive strength, and Young's modulus in the submitted manuscript could not fully...

08 May 2024 7,171 3 View

Protein Inhibition Denaturation Assay: No absorbance detected at 660nm?

Methods are based on Chel-Guerrero and colleagues'Phytochemical Profile, Toxicity, and Pharmacological Potenti... . We use a 1M Tris buffer with pH of 6.8, with Bovine Serum Albumin as the...

25 April 2024 8,802 0 View

The electronics structure of TiF4 and HfF4?

TiF4 and HfF4 are interesting materials due to their low melting temprature, and they maybe used in electronic devices. But the study about their electronic prpperties is very rare. Do you know...

28 March 2024 2,246 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View