Hi fellow researchers.
I am seeking help in finding concentration ranges for chemotherapy drugs (CP, DOC, 5FU etc.) for in vitro assays in oral cancer cell lines. From my literature search, I have found that different papers use different ranges, but why and how is this so? I have read that this is based on the Cmax of the drug, but I still do not understand and can't find a standard range of concentrations. Can anyone guide me, or recommend a paper/manual/guideline on drug concentrations to use in in vitro drug assays?
Hello Omama Syed
The concentration range will depend on the compound, cell line, and experimental conditions. Since it is not feasible to cover such a large span of concentrations with relatively fine spacing needed for accurate curve fitting (say about 3-fold difference from one concentration to the next), it is recommended that you run a trial experiment to determine the approximate range of drug sensitivity for the cell line under study with 10-fold spacing in drug concentration. Dose ranging studies may be followed by analysis of more narrowly spaced concentrations around the responsive range.
If the dose response datasets are available, you could look up for an appropriate concentration range for the cell line of interest. If you fail, then you follow the standard approach namely, to perform a 9-point dose-response assay ranging from 1nM to 10μM with half-log10 steps.
I have attached a few references that may be helpful.
Article Abstract 5502: Metrics other than potency reveal systematic ...
Chapter Measuring Cancer Drug Sensitivity and Resistance in Cultured...
Article Addendum: The Cancer Cell Line Encyclopedia enables predicti...
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