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Questions related from Omama Syed
Hello everyone. I am working on in vitro drug assays on cancer cell lines, which I will analyze using cytoxicity assays (MTT + SRB). However, my lab lacks access to a microplate reader, so I...
04 December 2024 8,243 1 View
Hi fellow researchers. I am seeking help in finding concentration ranges for chemotherapy drugs (CP, DOC, 5FU etc.) for in vitro assays in oral cancer cell lines. From my literature search, I have...
12 September 2024 6,228 1 View
I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...
13 May 2024 9,347 2 View
Hi everyone, I’m reaching out to the cell biology community to gather insights on cell viability assays. With a variety of options available—MTT, XTT, WST-1, SRB, LDH, Neutral Red,...
01 January 1970 3,093 16 View
Hello everyone. I am testing drugs at various concentrations against OSCC cells using in vitro MTT assays. Our lab lacks a plate reader, so we use a UV-Vis spectrometer compatible with a 96-well...
01 January 1970 4,821 7 View
Hi all. I hope to learn which companies' (sigma, thermo scientific, etc.) cell viability kits or reagents are popular for cancer research. Specifically, I would like to know about MTT, CCK-8,...
01 January 1970 9,096 1 View
Hi all. My lab is starting chemotherapy drug sensitivity assays for oral cancer cell lines. We will be detecting cell viability with the MTT and SRB assays, both of which are fluorometric. Hence,...
01 January 1970 4,460 3 View
Hello everyone. I am addressing the cancer researchers here who have worked on oral cancer cells or cell culture using OSCC cell lines. Which cell viability assay do you recommend for drug assays...
01 January 1970 3,902 2 View
Hello everyone. I want to reach out to the cell biology/cancer researchers and ask for their advice on drug assays against oral squamous cell carcinoma. My lab is going to launch chemotherapy drug...
01 January 1970 1,595 0 View