Omama Syed

9 Questions 3 Answers 0 Followers

Questions related from Omama Syed

Can Cytotoxicity Assay Plates Be Transported for Analysis Without Compromising Results?

Hello everyone. I am working on in vitro drug assays on cancer cell lines, which I will analyze using cytoxicity assays (MTT + SRB). However, my lab lacks access to a microplate reader, so I...

04 December 2024 8,254 1 View

How do I set a concentration range for my chemotherapy drug sensitivity assay?

Hi fellow researchers. I am seeking help in finding concentration ranges for chemotherapy drugs (CP, DOC, 5FU etc.) for in vitro assays in oral cancer cell lines. From my literature search, I have...

12 September 2024 6,236 1 View

Cell Culture Debris? or Contamination?

I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...

13 May 2024 9,364 2 View

Seeking Your Insights: Which Cell Viability Assay Do You Prefer and Why?

Hi everyone, I’m reaching out to the cell biology community to gather insights on cell viability assays. With a variety of options available—MTT, XTT, WST-1, SRB, LDH, Neutral Red,...

01 January 1970 3,099 16 View

Guidance on MTT Assay in 6-Well and 96-Well Plates

Hello everyone. I am testing drugs at various concentrations against OSCC cells using in vitro MTT assays. Our lab lacks a plate reader, so we use a UV-Vis spectrometer compatible with a 96-well...

01 January 1970 4,828 7 View

Which brand's cell viability assay kit/reagent do you use or prefer for cancer cell culture?

Hi all. I hope to learn which companies' (sigma, thermo scientific, etc.) cell viability kits or reagents are popular for cancer research. Specifically, I would like to know about MTT, CCK-8,...

01 January 1970 9,100 1 View

Which microplate reader do you recommend for drug sensitivity assays?

Hi all. My lab is starting chemotherapy drug sensitivity assays for oral cancer cell lines. We will be detecting cell viability with the MTT and SRB assays, both of which are fluorometric. Hence,...

01 January 1970 4,466 3 View

Which cell viability assay would you recommend for human oral cancer?

Hello everyone. I am addressing the cancer researchers here who have worked on oral cancer cells or cell culture using OSCC cell lines. Which cell viability assay do you recommend for drug assays...

01 January 1970 3,908 2 View

Which cell viability assay do you recommend for human oral cancer?

Hello everyone. I want to reach out to the cell biology/cancer researchers and ask for their advice on drug assays against oral squamous cell carcinoma. My lab is going to launch chemotherapy drug...

01 January 1970 1,598 0 View