How do I separate SSR products?

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Mostafa Haghpanah

dear Nemati

I saw in few excrements of SSR markers use of agarose gel with high density was useful, but it depended on your polymorphic locus.

I recommend to you ,use polyacrylamide gel.

Diana Duarte-Delgado

You might take a look to spreadex gels that are ready to use gels with resolution of 4pb

https://www.biolynx.ca/pdf/admin/el_spreadexfl.pdf

Polyacrylamide gels even though, have the best resolution when there are alleles with differences of less than 4 pb.

Zahra Nemati

Do You have a protocol for polyacryamid gel?

yes I have.

please give me e-mail address for send protocol to you.

maybe a few days late.

Thank you very much, no problem,

[email protected]

Taghi Ghassemi-Khademi

For my opinion, Metaphore agarose is very suitable for SSR markers and It is very safe and fast method than Acrylamide Gel. If you note in papers, in several study, have been used from SSR markers.