What is a good reference on designing experiments to determine molecular mechanism of disease or biological process?

I need to learn techniques and strategies in planning experiments that try to figure out the molecular mechanisms. Thanks.

01 January 2021 2,525 3 View

Has anybody had staining problems with lysotracker in live cells?

I am working with two fish cells types, a zebrafish liver cell line and primary cultures of trout macrophages. We are using lysotracker to stain them in order to visualize lysosomes in confocal...

16 December 2020 3,935 2 View

Is TRIS-EDTA-CITRATE buffer (pH 7.8) a good antigen retrieve for the TUNEL assay?

Hello everyone! I performed a TUNEL assay simultaneously with an immunofluorescence (SOX9 antibody) on the same sections of human testis. The fluorescence of TUNEL turned out well, but that of...

15 December 2020 1,270 3 View

Does a manager's background such as culture and attitude influence the performance of employees?

30 November 2020 1,585 2 View

If I want to use mean as calculation for my questionnaire, should I test the reliability and validity?

I'm using questionnaire for my research and mean (average) as my data analysis. I'm wondering how to calculate the reliability and validity of my data.

28 September 2020 3,517 3 View

How to induce with L-arabinose Chaperone Competent Cell BL21 (Takara)?

I am using Chaperone Competent Cell BL21 from Takara, pGro7/BL21 , but it is not clear on the protocol how and when I should induce the expression of the chaperones with L-arabinose. First time I...

14 September 2020 8,488 3 View

Is it better to use the arithmetic mean or geometric mean when averaging MPN?g or CFU/g measurements?

This data is from a microbiological evaluation of streetfood locally sold in our region. Thanks.

12 August 2020 5,981 3 View

Any recommendations on the software to do text mining or sentiment analysis on Chinese reviews?

I am trying to do text mining on Chinese reviews. I have tried out many softwares, like the RapidMiner, Chinese Text Analytics, Python. Most of them seem to require certain level of programming...

12 August 2020 9,109 8 View

Where can I find a good set of microbiological standards for aerobic plate count and coliform levels for street food or ready to eat food?

I need these to evaluate the data I obtained from my study. Thanks

10 August 2020 7,466 12 View

What is a good Thomson Reuters or Scopus indexed journal where I can publish my paper regarding Microbial safety aspects of street foods?

I need a journal with a faster turn around time, reasonable review process, and preferably no publication fees.

15 July 2020 8,241 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

Differential equation for anaerobic digestion proccess ?

Could anyone give me an example of a partial differential equation, along with the appropriate boundary conditions, that can be used in modeling a semi-continuous anaerobic digestion process? I'll...

25 November 2020 7,226 3 View

Would you suggest me a formula for calculating energy yield from biohydrogen and biomethane production?

I was looking for a formula to calculated the obtained energy yield via biohydrogen and biomethane production using anaerobic digestion process. The unit is in SI unit (x) mLH2/g VS and (y) mL...

20 October 2020 8,668 5 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

What is the best way to prepare anaerobic medium?

Hi, I would like to prepare anaerobic medium for cultivation of gut microflora. I have an anaerobic chamber. How should I remove oxygen from the medium? Is it enough to leave it for 48 hours in...

05 October 2020 3,093 4 View