Irene Feliciotti

7 Questions 2 Answers 0 Followers

Questions related from Irene Feliciotti

How do I purify a protein from cell lysate without affinity chromatography?

I have liters of cell lysate and I need to separate the recombinant protein from the rest. The volume is very large for the affinity chromatography column. Any other technique? I don't need high...

29 September 2023 4,173 4 View

What about the industrial use of BL21? Is it allowed? What is the best bacterial for large scale enzyme production?

I hope someone can help me with this! I am currently working with one enzyme expressed in E. coli. We want to scale up the process to an industrial production (no Academia involved). The...

27 September 2023 3,646 1 View

How do I revive frozen Lactobacillus rhamnosus?

I recently requested a Lactobacillus rhamnosus strain from ATCC and it will be delivered frozen. But I don't know the protocol to revive it and they can't provide that technical information....

31 January 2022 4,060 2 View

Any pNPG activity assay protocol available?

I want to test Beta-galactosidase (purified protein) activity using pNPG. I have always used oNPG. There is any difference? Should I change the buffer or I can use Sodium Phosphate? Should I...

18 August 2021 9,964 0 View

Best crystal dye for crystal or salts screening (in UK)?

Hello, I am new to Crystallization and I am looking for a trick to identify protein crystals or salts in my samples. Does anyone know a dye that I can use for this kind of screening? This has to...

21 July 2021 2,393 4 View

How can I test bile salt hydrolases with colorimetic assay kit?

I need to clone and purify 3 different BSH and I need to know if these are still active after the purification step. A would like to have a quick qualitative and quantitative colorimetric assay...

14 April 2021 449 1 View

How to induce with L-arabinose Chaperone Competent Cell BL21 (Takara)?

I am using Chaperone Competent Cell BL21 from Takara, pGro7/BL21 , but it is not clear on the protocol how and when I should induce the expression of the chaperones with L-arabinose. First time I...

15 September 2020 8,688 3 View