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Questions related from Irene Feliciotti
Hello Everyone! I need to find an easy and clean way to test bacterial contamination. I was thinking about using the API20E strips or the Enterotube, but I can't find a way to purchase these. Does...
24 March 2025 4,593 1 View
Hello, I am trying to screen a few recombinant proteins in a 48-well and 96-well plate format. I need to purify those proteins with Ni-NTA resin but the customised plates are too expensive. Does...
10 October 2024 7,514 1 View
I have liters of cell lysate and I need to separate the recombinant protein from the rest. The volume is very large for the affinity chromatography column. Any other technique? I don't need high...
29 September 2023 4,238 4 View
I hope someone can help me with this! I am currently working with one enzyme expressed in E. coli. We want to scale up the process to an industrial production (no Academia involved). The...
27 September 2023 3,699 1 View
I recently requested a Lactobacillus rhamnosus strain from ATCC and it will be delivered frozen. But I don't know the protocol to revive it and they can't provide that technical information....
31 January 2022 4,111 2 View
I want to test Beta-galactosidase (purified protein) activity using pNPG. I have always used oNPG. There is any difference? Should I change the buffer or I can use Sodium Phosphate? Should I...
18 August 2021 10,006 0 View
Hello, I am new to Crystallization and I am looking for a trick to identify protein crystals or salts in my samples. Does anyone know a dye that I can use for this kind of screening? This has to...
21 July 2021 2,503 4 View
I need to clone and purify 3 different BSH and I need to know if these are still active after the purification step. A would like to have a quick qualitative and quantitative colorimetric assay...
14 April 2021 499 1 View
I am using Chaperone Competent Cell BL21 from Takara, pGro7/BL21 , but it is not clear on the protocol how and when I should induce the expression of the chaperones with L-arabinose. First time I...
15 September 2020 8,776 3 View
I am currently working with one enzyme expressed in E. coli. We want to scale up the process to an industrial production (no Academia involved). The regulation of E. coli BL21 cells is not clear...
01 January 1970 471 2 View
