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Questions related from Irene Feliciotti
I have liters of cell lysate and I need to separate the recombinant protein from the rest. The volume is very large for the affinity chromatography column. Any other technique? I don't need high...
29 September 2023 4,172 4 View
I hope someone can help me with this! I am currently working with one enzyme expressed in E. coli. We want to scale up the process to an industrial production (no Academia involved). The...
27 September 2023 3,645 1 View
I recently requested a Lactobacillus rhamnosus strain from ATCC and it will be delivered frozen. But I don't know the protocol to revive it and they can't provide that technical information....
31 January 2022 4,060 2 View
I want to test Beta-galactosidase (purified protein) activity using pNPG. I have always used oNPG. There is any difference? Should I change the buffer or I can use Sodium Phosphate? Should I...
18 August 2021 9,963 0 View
Hello, I am new to Crystallization and I am looking for a trick to identify protein crystals or salts in my samples. Does anyone know a dye that I can use for this kind of screening? This has to...
21 July 2021 2,390 4 View
I need to clone and purify 3 different BSH and I need to know if these are still active after the purification step. A would like to have a quick qualitative and quantitative colorimetric assay...
14 April 2021 448 1 View
I am using Chaperone Competent Cell BL21 from Takara, pGro7/BL21 , but it is not clear on the protocol how and when I should induce the expression of the chaperones with L-arabinose. First time I...
15 September 2020 8,681 3 View
