How do I resuspend lentivirus after concentrating with Lenti-X concentrator?

24 January 2025 1 9K Report

The pellet is very sticky and will not go into solution. Can I vortex it? I need to add ~1ml of media to a pellet made from 10ml of lentil-X to get it to go moderately into suspension.

Abhijit Basu

Dissolve the pellet with the same fresh media (100-500ul) that you used to grow your lenti cells. store at 4 degree and use within one week. For longer storage at -80 degree use cell freeze media.

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