Does Autoclaving and gamma irradiation remove DNA enough so that no DNA fragment will be amplified in PCR?
Autoclaving to do not completely remove DNA and irradiation method has its own limitations and not efficient for longer DNA fragments.
You can go through the comparison of different decontamination methods, it might help you to decide your method of choice.
Wu, Y, Wu, J, Zhang, Z, Cheng, C. DNA decontamination methods for internal quality management in clinical PCR laboratories. J Clin Lab Anal. 2018; 32:e22290.
Fischer M, Renevey N, Thür B, Hoffmann D, Beer M, Hoffmann B. Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories. PLoS One. 2016;11(7):e0159274. Published 2016 Jul 13. doi:10.1371/journal.pone.0159274
Kaye N. Ballantyne, Renato Salemi, Fabio Guarino, James R. Pearson, Dale Garlepp, Stephen Fowler & Roland A.H. van Oorschot (2015) DNA contamination minimisation – finding an effective cleaning method, Australian Journal of Forensic Sciences, 47:4, 428-439
- You did say DNA and RNA
- For DNA as described
- For RNA 3% hydrogen peroxide or water with 0.1% DEPC (not autoclaved) will work; as will 10% SDS
- Ideally any glassware for RNA work should be baked at >180C for 5 hours but if that isn't possible using hydrogen peroxide and or unautoclaved DEPC water followed by sluice with autoclaved DEPC water will be fine
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