auxillary node culture
i used 3 mg/l of cefataxime sodium salt
can i use antifungal agents in media? if so what?
In experiments with sugarcane stem buds, we usually employ treatments with Sodium hypochlorite solution 2% for 30 minutes, followed by wash in Ethanol solution 70% for 30 seconds, and including washes in sterile destilled water for 3 times after each treatment. If the nodes are too big, the time with Na hypochlorite and Ethanol can be extended in the same proportion.
I agree with Dr. Fluminhan . you can also treat your explants using mercuric chloride *(0.1-0.2%) followed by 70% ethanol and finally washing with autoclaved distilled water for 3- 4 times.
About the antifungal agents ; yes you can use. there are many like carbendazim, fenbendazole, miconazole and imazalil which are relatively safe and also have a broad spectrum of antifungal activity. Also I would recommend you to use PPM (plant preservative mixture) [0.1-0.2%] as it is a broad-spectrum preservative and biocide, which kills bacteria and fungi cells, prevents germination of spores, and in higher concentrations, can eliminate endogenous contamination in explants. Also it is heat stable and can be autoclaved with media.
Regards
Shiwani Kaushal
To reduce fungal contamination use Bavastin. i.e. washy your seed or plants with Bavistin.
To reduce bacterial contamination wash ut seed or plant with 2 x antibiotic solution.
If the seed coat is strange use fumigation method to sterilize your seed in desiccator ( ml Sodium Hypo-chloride and 3ml con HCl in desiccator for over night.
What would be the antibiotic solution suitable for bacterial contamination? The method should be easier to use I believe. Therefore, it is important to identify a commonly available antibiotic and the strength of the solution, i.e. mg/liter, should be determined. Appreciate your experience on this.
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