Hello,
might be a silly question but I am a confused about how to do this, and want to make sure I get it right.
I have a vial with 5 mg of Verteporfin, the zigma data sheet says the product has a solubility in DMSO of 2mg/ml but this would require me to add 2.5 L of DMSO. On the other on the tocris website it says that Verteporfin has a max solubility in DMSO of 14.38 mg/ml for a max solubility of 20mM which I can actually work with. I would like to reconstitute the drug to stock solution in the vial which I can then add to a 12 well plate at concentrations of 2, 5 10, and 20uM. how do I go about this? Will the drug precipitate out if I when I add it to cell media? Thank you for in advance for any help
Best
Dear Andres Stucky
If tocris website was right about the solubility, the maximum concentration you can have is 20mM so I think you should prepare the solution at this maximum concentration since you want to work with at one of your test which you use the same concentration for treatment. Add 5mg of the compound in 347.71 ml DMSO, you'll have a solution of 20mM, but I guess you dont need this volume of the solution! So try only 0.2 mg of the drug in 13.9 ml DMSO, considering the lower concentrations you need for other wells, you need to dilute the solution further, so dont worry about the low volume of the stock solution. If you could tell the exact volume of the solution you want to add to every well and the volume of the cell media, I would give the more precise values; however, be careful that the volume of the cell media will dilute your drug (especially if the drug is highly soluble in water), so you are going to have to consider the volume of cell media in every well in your calculations. Moreover, keep an eye on the volume of the cell media to keep the cells fully nourished and prevent them from the damage of DMSO.
I hope this helps
Good luck!
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