How do I purify rabbit IgG4?

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Engelbert Buxbaum

Purification of immunoglobulin from serum is straight forward with either ammonium sulphate or PEG. IgG can be purified from the immunoglobulins with protein G (more specific than protein A). However, separating IgG4 from all other immunoglobulins will require affinity purification of the gamma-4 component. If anti-human IgG4 antibodies can be used for rabbit IgG4 only experiments can tell.

Nkemngo Francis Nongley

Hi Sir. Can u please read the articles attached and adapt a harmonise protocol from it for the IgG4 antibody purification. I hope it helps.

Robert Adamu Shey

Thanks Yi Wan

and Engelbert Buxbaum and Nkemngo Francis Nongley . I will follow your responses.