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Good day! Pleas can anyone help me with a protocol for site directed cloning of insert DNA containing XhoI/BamHI restriction fragments on PET15b expression vector containing XhoI/BamHI sites...
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I cloned Cryptosporidium parvum LDH gene using PMAL-C2X plasmid for which I had clear colonies after transformation on Amp+ plates. After performing miniprep of some selected colonies, I didn't...
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