Nkemngo Francis Nongley

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Questions related from Nkemngo Francis Nongley

Site directed cloning of DNA insert in PET15B VECTOR ?

Good day! Pleas can anyone help me with a protocol for site directed cloning of insert DNA containing XhoI/BamHI restriction fragments on PET15b expression vector containing XhoI/BamHI sites...

03 March 2018 8,292 5 View

Good day, Why post- transformation restriction enzyme digestion didnot give me bands of my plasmid and insert?

I cloned Cryptosporidium parvum LDH gene using PMAL-C2X plasmid for which I had clear colonies after transformation on Amp+ plates. After performing miniprep of some selected colonies, I didn't...

03 March 2018 7,000 4 View