hi. TBE buffer: 4ml EDTA 0.5M with PH8, 5.5gram boric acid and 10.8 gram Tris Base
TE buffer: 1ml Tris 1M with PH:7.5, 200 macroliter EDTA 0.5 M with PH 8 then extend volume to 100cc with distilled water
hi. TBE buffer: 4ml EDTA 0.5M with PH8, 5.5gram boric acid and 10.8 gram Tris Base
TE buffer: 1ml Tris 1M with PH:7.5, 200 macroliter EDTA 0.5 M with PH 8 then extend volume to 100cc with distilled water
TBE buffer (5X) stock solution per litre- 54g of Tris base; 27.5g of boric acid and 20ml of 0.5M EDTA (pH 8.0)
1X TE buffer (pH 8.0)- 1M Tris (pH 8.0, 1 ml), 0.5M EDTA (pH 8.0, 200 μl), makeup volume to 100ml with DDW.
(Source- Molecular cloning a laboratory manual by Sambrook and Russell, vol III appendix section)
TBE electrophoresis buffer (10X)
Reagent Quantity (for 1 L) Final concentration
Tris base 121.1 g 1 M
Boric acid 61.8 g 1 M
EDTA (disodium salt) 7.4 g 0.02 M
Prepare with RNase-free H2O. Dilute 100 mL to 1 L to make gel running buffer. Store for up to 6 mo at room temperature.
TE buffer
10 mM Tris-Cl, pH 7.5
1 mM EDTA
Make from 1M stock of Tris-Cl (pH 7.5) and 500 mM stock of EDTA (pH 8.0).
10ml 1M Tris-Cl pH 7.5 per L
2ml 500mM EDTA pH 8.0
1M Tris (crystallized free base)
Tris(hydroxymethyl) aminomethane
FW 121.4 g/mol
60.57 g in 0.5L mq water
pH to 7.5 using HCl
0.5M EDTA
Diaminoethane tetraacetic acid
FW 372.2 g/mol
18.6 g in 100ml mq water
pH to 8.0 using NaOH
• EDTA will not be soluble until pH reaches 8.0
• Use vigorous stirring, moderate heat (if desired) and time
https://www.thebalance.com/how-to-make-tbe-buffer-in-3-easy-steps-375493
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