i need to use carbonate buffer, but there are different procedures. please explain the standard procedure or please give me the good reference.
Best methods for buffers are found as appendix in
Pharmacopoeas ( B. P. or USP P); unfortunatly I can't upload or attach it.
Preparation of Bicarbonate-Carbonate Buffer Solutions (pH 9.12–10.83)
To create 100ml of a 0.1M bicarbonate buffer solution, mix sodium bicarbonate and sodium carbonate decahydrate, as given below.
Solution A: 0.1M sodium bicarbonate (NaHCO3 MW = 84.0) (8.40 g/L)
Solution B: 0.1M sodium carbonate decahydrate (Na2CO3.10H2O MW = 286.2) (28.62 g/L)
References:
Delory & King, Biochem. J., 39, 245 (1945).
D. D. Perrin, Boyd Dempsey - Buffers for pH and Metal Ion Control - Springer Netherlands (1974).
http://www.sigmaaldrich.com/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html.
Please find the easiest combination here..
http://cshprotocols.cshlp.org/content/2011/2/pdb.rec12398.full?sid=e4b44f93-e28d-4272-af47-1f70216a8bc4
@ Luciano de Almeida Pinheiro Good Link :)
For the acidic pka, Anahita, the acid/base conjugates are carbonic acid H2CO3/Hydrogen carbonate HCO3-. The same equation would apply: pH = pka + log ([base]/[acid]). But, carbonic acid exists only in its sodiated forms. So only way is to titrate a sodium bicarbonate solution with HCl. Keep track of how much NaCl you're generating and keep in mind that bicarbonate anion cannot be constantly maintained at acidic pH because it would slowly decompose to water and carbon dioxide.
Can ammonium carbonate buffer be used in place of ammonium bicarbonate buffer?
How to prepare 25mM of sodium carbonate-bicarbonate buffer with pH 10.6?
Luciano de Almeida Pinheiro
For related RG discussions; see: https://www.researchgate.net/post/How_can_stabilise_the_pH_of_01M_sodium_bicarbonate_solution_made_in_the_lab_to_80 https://www.researchgate.net/post/Anyone_know_how_to_prepare_2_M_of_sodium_bicarbonate_pH_100_solution_no_buffer
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH