I am doing patch clamp recording from neurons and at the end I extract the intracellular content for transcriptomic profiling. I know RNA is subjected to a fast degradation and a quality control is very important especially at the beginning while still establishing the method. I was suggested to use a simple and minimalistic lysis buffer for RNA extraction that basically contains water, Triton and RNAse inhibitor. I was wondering how can I perform a quality control without using some sort of standardized external RNA like ERCC.
*Are there other types of quality control that can be performed even without ERCC, like for example by detecting the already known transcripts during library alignment?
*And what is the best way to perform a quality control for single cell RNA extraction and transcriptomics?
Thank you in advance for any help!!
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