How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

08 July 2020 8,434 2 View

Who does believe that Remdesivir has to be used early on and not (only) in advanced cases of SARS-Cov-2?

Remdesivir has been shown to speed up recovery times for patients with COVID-19 in a major US-led trial, becoming the first drug with proven benefit against the disease. Now, considering the...

01 May 2020 4,105 2 View

How can I get rid of spam on Google Scholar's "Recommended articles"?

I used to like the "Recommended articles" function in Scholar. It recommends you articles based on the citations you received (you can access them below the scholar search bar, and receive them as...

21 April 2020 6,221 7 View

If female mice are put together on the same cage, are all of them on the same estrous cycle stage?

I work with female mice and I have this doubt. If they are all put together on the same cage, are all of them on the same estrous cycle stage? If so, is there any literature that supports...

01 April 2020 6,266 10 View

How to blast a paired end fastq file?

Hello everyone, I have a paired end fastq file and I know that BLAST+ in command line, accepts fasta format. But I don't know how does it work for a paired end fastq file (I mean in two different...

22 May 2019 2,482 2 View

260/230 ratio of my RNA samples are very low. Is there any method to try purify the sample and synthesize cDNA!?

I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR. I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios...

16 April 2019 768 2 View

How to perform KASP genotyping of zebrafish larvae at 72hpf?

Dear all, I need to genotype some zebrafish larvae at 3-4dpf. I currently tried to perform it as I do it for the adults, but it did not work. Basically: 1. Fin clip and add the fin to a 96 well...

20 January 2019 989 3 View

IHC in peripheral blood smear?

Hi everyone. We want to do an IHC in peripheral blood smear but it's our first time, and we have some doubts. How do we have to fix the samples? Which is the best fixation agent, acetone, methanol...

15 October 2018 9,338 5 View

Non-transcriptomic pathways that can increase lipid accumulation?

I am currently doing a project investigating the lipid accumulation potential of a chemical using an in vitro model. Upon observing significant lipid droplet accumulation at various...

18 March 2018 4,812 4 View

Are you studying affects toward science, technology and/or mathematics in university and preuniversity students in your country?

We may share instrument and compare data with ours.

02 January 2018 1,064 12 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Could one suggest a common formula for Penicillium Chrysogenum spore counting by spectroscopy? and also its nm?

I am working with this strain but I do not have the formula for spore counting. please just suggest a spectrophotometric method.

05 January 2021 4,308 3 View

Is this method mentioned for sporulation assessment reliable ?

I had come across a protocol to assess and score the sporulation of fungi, where they has taken out a 5 mm fungal disc and transferred to 5ml sterile dist.water and spore count was recorded under...

05 January 2021 9,468 1 View

CFU treatment log reduction without control?

Hello all, I am trying to create a graph on excel to express -log10 reduction of treatment groups and CFU spores using the equation -log10(final concentration/initial). However, one of my groups...

28 November 2020 6,074 3 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

How do I remove Bacillus sp. spores from Kraft Paper machine?

In the pulp and paper company that I work in, samples from paper, water and pulp from the paper machine gave information about existing spores of Presumptive Bacillus cereus. Our paper is used...

03 November 2020 1,726 10 View

How to isolate Clostridium from mixed culture?

My Clostridium tyrobutyricum seems not producing spores. I incubated the Clostridium tyrobutyricum in 10 ml of RCM at 28 degrees Celcius for 5 days anaerobically, then heat the whole tube in 75...

25 October 2020 3,386 1 View

How does the spores of Bacillus anthracis cause the anthrax disease and how can it be weaponized?

Anthrax is a disease caused by aerobic spore-forming Bacillus anthracis. This spore can resist environmental changes and resist biological extremes of heat, pH, chemicals, and other factors; it...

20 October 2020 4,658 8 View