Raquel Neri

5 Questions 9 Answers 0 Followers

Questions related from Raquel Neri

How do I know the number of Streptomyces spores that are in my sample?

Hello everyone! I'm conducting intergeneric conjugation experiments between Strepotmyces and E. coli, but in all protocols they say that I have to have a determined amount of Streptomyces spores....

25 August 2020 767 2 View

How can I know which family/species of virus is making the difference between my samples?

I have several metagenomic samples, from which I got the viromes through a bioinformatics pipeline. I made a PCoA of those samples, and some of the samples cluster together. I would like to know...

09 July 2020 8,573 2 View

Does anybody know what does this warning mean when running permanova in R (adonis)?

I have an abundance matrix with samples and OTU's present in those samples. I want to know if the samples are different between each other, so I ran a PERMANOVA in R with default options, using...

30 January 2020 7,033 3 View

How to blast a paired end fastq file?

Hello everyone, I have a paired end fastq file and I know that BLAST+ in command line, accepts fasta format. But I don't know how does it work for a paired end fastq file (I mean in two different...

23 May 2019 2,615 2 View

Someone has experience with conjugation between E. coli and Streptomyces?

Hello, I´ve been trying to transfer a 11 kb plasmid (pCRISPomyces ADDGENE) from E. coli ET12567 to Streptomyces mobaraensis. I have done it three times already with two different methods and I...

01 January 1970 533 4 View