I know the strain itself is, but does that imply that it is due to the F' plasmid?
The genotype of ER2738 is
F´proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10(TetR)/ fhuA2 glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5.
Tn10 which encodes Tetracycline resistance is inserted into the F' plasmid. If your sample of ER2738 is Tet-R, then it is likely that it has an intact F' plasmid.
Supporting the above two information.. you can test it by just do a plasmid mini preparation and transform perform transformation of tetracycline sensitive strain (any common E. coli cell). If they can grow on tetracycline plate...you proved that the plasmid had the antibiotic resistance gene (tetR).
Good Luck
Paul, thank you! My understanding was that tetracycline is used to select for those with F' plasmids, but I wasn't sure. Does the "Tn10" portion mean the gene is located on the F' plasmid? How can you tell from the genotype?
I agree with the answer of Mr. Paul and Mr. Deepak, you do not need to hesitate because of antibiotic resistance genes in plasmids is not in the chromosome
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