Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What would cause different shape DMA loss modulus curve in different batches of PC+ABS?

I have some DMA curves of PC+ABS from different batches. The PC loss modulus peak is fairly consistent but one batch has a peak value 10degC lower than the rest. Another peaks at the normal value,...

06 December 2020 809 2 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

Phosphomimetic Peptides & Phospho-Specific Antibodies?

I am performing experiments using synthetic cell-penetrating peptides in acute hippocampal slices. Our lab has verified the NLS-mediated nuclear delivery of a number of such peptides in the region...

27 December 2019 7,914 2 View

What is the recommended fixation time of 400 um hippocampal slices in 4% PFA?

I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...

22 June 2018 9,560 5 View

How long does Apex Taq RED Master Mix, 2X remain viable after opening?

The manufacturer's data sheet says the unopened vial lasts for 2 years at -20 C and that the mix is stable at 4 C for at least six months. The dye we are using has been opened and has been kept at...

10 June 2018 3,902 3 View

Is agitation necessary during IHC antibody incubation for 400 um hippocampal slices?

I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid...

09 June 2018 9,562 3 View

Is it normal for signal intensity to fluctuate during chemiluminescent detection with Versadoc?

I've noticed this strange phenomenon where my signal intensity will change from picture to picture over the course of my exposure. For example: I will expose membrane to ECL for 2 min, signal...

13 February 2018 1,529 2 View

How to use McNemar's test to compare accuracy of classifications?

Hi, I have seen various studies use McNemar's test to statistically test the accuracy of several classifications. I have six classifications, produced using three different classifiers (SAM, SVM,...

19 September 2017 5,034 4 View

What is the duration of kanamycin selection pressure in common KanR-expressing E. coli LB cultures?

I have used Kanamycin resistance as a selection marker with kanr-containing pET vectors. Since KanR is inactivating the antibiotic (only in the cytoplasm), I was wondering about the duration of...

15 April 2015 8,463 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View