How do I go about validating DNA methylation and expression levels of miRNAs that were differentially methylated in my whole methylome study?

27 February 2017 1 5K Report

For analysis of DNA methylation, I was thinking a normal bisulfite PCR would do, using primers designed on the miRNA flanking regions. However, I have not found any literature in which such validation has been carried out. Also, for the expression analysis, should I use the Taqman probes designed for precursor miRNAs or is it better to validate the mature miRNAs?

Ross L Tellam

BS-PCR or BS-sequencing are the gold standards for DNA methylation validation.

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