I want to reduce metal contents of soil by introducing certain microbes in order to precipitate out metals and quantify. But I dont know how to go about it. Can someone help me out!!!!
The best way to measure the total metal content in your soil sample is by digestion, and then takes another sample and measure the metal content in the leachate water. Finally, calculate the difference between the total metal content and the leachate metal content. The difference represent the reduced metal content by microbe.
I will suggest go through the following publications for techniques to handle the microbes to reduce the metal contents in the soil samples (full text available online). Actually, it depends on metal availability in your soil samples and selection of bacterial species. Also design of your experiment and techniques, in situ or ex situ bioremediation or the use of anaerobic or aerobic bioreactors, etc.
1. Ahmed M., Malik A., 2011. Bioaccumulation of heavy metals by zinc resistant bacteria isolated from agriculture soils irrigated with wastewater. Bacteriology J, 2: 12-21.
2. Rajendran P., Muthukrishnan J., Gunasekaran, P., 2003. Microbes in heavy metal remediation. Indian J. Experimental Biology, 41: 935-944.
3. Kumar. A, Bisht. B. S, Joshi. V. D, Dhewa. T., 2011. Review on bioremediation of polluted environment: a management tool, International Journal of Environmental Sciences. 1: (6), 1079-1093.