How do I find out the flanking ends or the complete genome sequence of a given sequence that I have obtained from a PCR product?

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My data for fungicide treated replication shows higher RMSE and R-square but less CV compared to the untreated replication for a regression between vegetation indices and yield. How correct is it?...

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Where can I get CDS location for COVID-19 sequences of NCBI carona virus database?

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How to calculate sensitivity and specificity using Genscan Tool?

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30 December 2019 2,083 3 View

Is it necessary to have a reference electrode for EIS if the counter electrode is sufficiently large?

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Scalogram for huge data?

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May I know the difference between absolute reflectance and reflectance(transmittance) in ASD based measurements?

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How to statistically quantify sorghum grain yield loss by birds?

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Why there is no nitrogen in the reactions in Eddy Dissipation or Finite Rate/Eddy Dissipation?

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Hi im modelling a mass transport problem with a multimaterial domain ?

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How do I increase the yield of my PCR product?

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02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

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PACYC PCR not working after several attempts. Would anyone have any suggestions?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

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Runs of nucleotides in Sanger sequencing?

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Can thermal cycler machines be problematic during PCR?

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Calculating volume of DNA required from DNA concentration?

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26 February 2021 5,029 2 View

Karen A. Darbinyan Popular answer

Hello, see attached info.

Good luck!

Karen A. Darbinyan

Giorgia Pertile

Run in the gel and cut the correct band. After the quantification you send the concentration they will need to make the quantification.

Michael J. Benedik

What are the results when you perform a Blast search with your sequence? Does it identify perfect homologs or close ones?