Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

How can I digest the brain tissue in healthy mice as evaluating the apoptosis using flow cytometery by annexin V/propidium iodide (PI)?

We digested the brain tissue with Trypsin ( 1x) for 15 min (all steps were performed in cold conditions) and suspend the cells by pipetting up and down (3-4 times) to the single-cell suspension....

22 August 2020 5,611 4 View

Hi, is there anyone tell me or share me a link for downloading the template for "Cities" journal of Elsevier?

I went through the cities journal website but unluckily couldn't find the template

28 June 2020 811 3 View

Can anyone suggest a kit or protocol to extract Brucella spp. DNA from FFPE blocks?

Hi everyone! I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or...

15 December 2019 9,767 2 View

Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ?

Hello, Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ? Thank you

06 April 2019 8,782 19 View

What is the contribution of renewable energies in the fight against global warming?

The phenomenon of the greenhouse effect is a threat to the environment. What is the contribution of renewable energies in the fight against global warming?

04 April 2019 1,239 33 View

Methodology for implementing a thesis ?

Methodology for implementing a thesis involves: - State of the art of the studied phenomenon - Study context - Introduction - Hypotheses - Objectives - Labor interest - Methodology apprehended -...

14 February 2019 6,502 16 View

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

24 January 2019 5,584 2 View

How is 3D pelvic kinematics examined during walking before and after interventional exercises in patients with pelvic inclination?

in general, for healthy people, in the 3D motion analysis to define the anatomical coordinate system on segments, one frame in static position is taken by the camera, which will make segment...

10 October 2018 4,098 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View