Hello,
I am working with two strains of Pseudomonas aeruginosa, one being an isogenic strain which has show to produce significant mucus.
I have cultured both strains on nutrient agar, however, the mucoid strain grows like it's progenitor, similar to wild type Ps. aeruginosa.
Is there possibility of reversion on the agar, after 24 hours of culture at 30oC? or may it require more incubation time?
Thanks!
yes there is possibility of reversion. I personally have not worked with Pseudomonas. But organisms are kn own to revert to wild type when repeatedly grown on culture media.
Considering bmy experience I suggest passage through mice a few times or any other suitable animal species.
alginate production can be induced in low iron media
mBio 5(1):e01010-13. doi:10.1128/mBio.01010-13.
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