Where can I get Polyglycerol polyricinoleate (PGPR) as emulsifier for water in oil emulsion?

I am having difficulty to get polyglycerol polyricinoleate (PGPR) as surfactant in emulsion formulation. Currently, I am having problem in getting stable double nanoemulsion. I read most journals...

07 August 2019 710 1 View

How do I dissolve water in oil (w/o) nanoemulsion in water?

I did quick preliminary solubility study on my flavonoid enriched extract in oil and water phase. I found my extract is more soluble in water. Prior this study, I thought my extract is oil soluble...

06 July 2019 860 4 View

What are the companies producing active compounds i.e. flavonoids?

With regard to the question above, may I know any good companies are extracting, enriching or isolating natural active compounds i.e.flavonoids for pharmaceutical, nutraceutical or cosmaceutical...

04 May 2019 4,845 1 View

What is the alternative dye for Nile red (NR) for solvent polarity testing?

Hi. I would like to use this platform to ask question regarding the alternative dyes which can be used for solvent polarity testing. So far I came across Nile red (NR) which commonly used for...

04 May 2019 7,366 2 View

How do I evaporate off the natural deep eutectic solvent (NADES)?

I am currently developing natural deep eutectic solvents (NADES) for flavonoid extraction from my plant sample. However, I am having the difficulty to evaporate off the developed NADES in order to...

03 April 2019 3,749 5 View

Is it possible desorption ratio more than 100%?

Currently, I am conducting research on the adsorption and desorption of flavonoids in plant extract by using macroporous resins. After equilibrium, the adsorbed flavonoids by resins were treated...

09 October 2018 1,913 7 View

For dynamic adsorption studies, how do I know my adsorbent (resins) already reach equilibrium before I start desorbing it with solvent?

I am currently conducting dynamic adsorption and desoprtion studies on flavonoids by using macroporous resins. I am having difficulty to understand at what point should I consider the resins...

07 August 2018 5,920 2 View

How to separate very close peaks in HPLC?

I am having difficulty to separate very close peaks which are isomers. Currently I am using Xbridge Waters C18 column (4.5 mm, 150 mm and 5 micrometer). I used solvent A as water (0.1% acetic...

06 July 2018 3,004 4 View

Can Response Surface Methodology (RSM) be used after the optimization work already done?

I plan to analyze the optimization results using RSM. However, I wonder can my present data be used in RSM. Previously, I optimized my extraction conditions individually. For example, to get...

03 April 2018 345 3 View

Inconsistency of total flavonoid content (TFC) result?

I carried out TFC determination using NaNO2-AlCl3-NaOH assay in 96 well plates. However, after repeating a few times using the same methods and volume the result is inconsistent. I realised the...

03 April 2018 4,935 0 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View