Where can I get Polyglycerol polyricinoleate (PGPR) as emulsifier for water in oil emulsion?

I am having difficulty to get polyglycerol polyricinoleate (PGPR) as surfactant in emulsion formulation. Currently, I am having problem in getting stable double nanoemulsion. I read most journals...

07 August 2019 710 1 View

How do I dissolve water in oil (w/o) nanoemulsion in water?

I did quick preliminary solubility study on my flavonoid enriched extract in oil and water phase. I found my extract is more soluble in water. Prior this study, I thought my extract is oil soluble...

06 July 2019 860 4 View

What are the companies producing active compounds i.e. flavonoids?

With regard to the question above, may I know any good companies are extracting, enriching or isolating natural active compounds i.e.flavonoids for pharmaceutical, nutraceutical or cosmaceutical...

04 May 2019 4,845 1 View

What is the alternative dye for Nile red (NR) for solvent polarity testing?

Hi. I would like to use this platform to ask question regarding the alternative dyes which can be used for solvent polarity testing. So far I came across Nile red (NR) which commonly used for...

04 May 2019 7,366 2 View

How do I evaporate off the natural deep eutectic solvent (NADES)?

I am currently developing natural deep eutectic solvents (NADES) for flavonoid extraction from my plant sample. However, I am having the difficulty to evaporate off the developed NADES in order to...

03 April 2019 3,749 5 View

Is it possible desorption ratio more than 100%?

Currently, I am conducting research on the adsorption and desorption of flavonoids in plant extract by using macroporous resins. After equilibrium, the adsorbed flavonoids by resins were treated...

09 October 2018 1,913 7 View

How to separate very close peaks in HPLC?

I am having difficulty to separate very close peaks which are isomers. Currently I am using Xbridge Waters C18 column (4.5 mm, 150 mm and 5 micrometer). I used solvent A as water (0.1% acetic...

06 July 2018 3,004 4 View

Can Response Surface Methodology (RSM) be used after the optimization work already done?

I plan to analyze the optimization results using RSM. However, I wonder can my present data be used in RSM. Previously, I optimized my extraction conditions individually. For example, to get...

03 April 2018 345 3 View

Inconsistency of total flavonoid content (TFC) result?

I carried out TFC determination using NaNO2-AlCl3-NaOH assay in 96 well plates. However, after repeating a few times using the same methods and volume the result is inconsistent. I realised the...

03 April 2018 4,935 0 View

Can I use 570nm for FRAP?

I am doing research on FRAP (TPTZ reagent method) for antioxidant activity. I know the preferred wavelength is 593nm. Is it possible to measure at 570nm instead as for now the 593nm filter not...

02 March 2018 1,621 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View