24 June 2015 6 1K Report

Although running the DNA on agarose gel is the best method. Can absorbence be used for determining whether DNA is degraded or not? If yes than what is the criteria?

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How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

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What is the minimum number of cells required for fluorescence in situ hybridization?

I have to perform a FISH experiment with limited number of cells and I am trying to estimate the minimum number of cells required to do FISH. Cells are from mammalian cell line. I failed in doing...

12 January 2021 7,887 1 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Can someone suggest me a wear detection method for a pipe which goes under intense heat and pressure at intervals?

I am looking for a wear detection method for a pipe that goes under intense heat and pressure for short instances at intervals. this process has an average cooldown time of 8 seconds between each...

08 October 2020 3,776 2 View

Single-cell cloning from SkBr3?

Did anyone tried single-cell cloning from SkBr3 cell line. I am failing in growing cells post single cell seeding in wells. Any help is appreciated

27 September 2020 8,762 4 View

What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

We are getting some patients who were initially positive of RT PCR for Covid. But in retesting after 15-20 days or even 20 days after those patients remain still positive. Is it for dead RNA...

17 August 2020 9,552 9 View

How to calculate % sequence identity using Clustal W?

I am calculating % epitope conservation for different mammalian antibodies against zebrafish. I have performed the Clustal W for antigenic peptide used in raising the antibodies against the...

05 August 2020 6,399 5 View

What is the Source of Harmonic Power ?

Consider a sinusoidal supply at fundamental frequency as shown, with a series inductive reactance leading to a bus. Let a diode rectifier load be connected to the bus. The switch Sw for resistive...

24 June 2020 8,175 27 View

What is the best book on Game theory from the perspective of Computer Science and Engineering?

I do not know much about game theory. I am finding a book where different protocols and algorithms of computer science have been analyzed using the concept of game theory.

17 May 2020 4,774 7 View

How can a PhD in interdisciplinary fields can benefit a researcher in job market?

I am a PhD scholar of Humanities and my research is broadly connected to Anthropological studies and framework. I have also published with an Anthropological journal of global repute and looking...

10 May 2020 4,202 1 View

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Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

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How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

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Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

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Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

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LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

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