Debaashish Biswas

11 Questions 2 Answers 0 Followers

Questions related from Debaashish Biswas

Why cell growth after thawing of cell line is so slow?

I am using A549 cell line and i had made stocks in Nov 2017 and stored in -80. When I revived them back they are growing at a very slow pace. As doubling time in 22 hrs approx.But in this case I...

19 February 2018 3,092 2 View

Can anybody help me in providing chemical method for extraction of DNA from the cell line?

I want to extract DNA from A549 cell line for DNA fragmentation assay.

03 March 2017 511 2 View

Can anybody help me by providing me the detailed protocol of methanol fixation and giemsa staining used in colony formation assay?

Detailed protocol of Colony forming assay

11 December 2016 5,852 4 View

For MTT (cell viability assay), one should use round bottom or flat bottom 96-well plate?

Cell viability test with plant extract

27 October 2016 6,637 4 View

Once FBS being deactivated? One should deactivate FBS again before using it?

Suppose you have 15 ml falcon containing FBS. To prepare 50 ml media you require only 5 ml of FBS. Next day if you want to prepare another 50 ml media, then is it required to inactivate FBS again...

04 September 2016 7,870 3 View

Which kind of trypsin should be used while subculturing A549?

There are different categories of trypsin or trypsin-EDTA ? Which one to use? Which will give better results?

04 September 2016 2,057 1 View

Is a positive control in qPCR necessary to include or not?

Is it advisable to run a positive control with unknown samples in qPCR?

30 July 2015 1,735 5 View

Do gaps between the primer and probe affects real time amplification?

For EGFR mutation analysis in Exon 18 to 21 by real time we use single tube having primers, WT probe tagged with VIC, Mutation probe tagged with FAM, Mastermix and 1 microlitre DNA (both WT and...

05 July 2015 6,900 3 View

Is it possible to get EGFR Exon 18 mutations more than the expected rates?

Like the reported rate for the same is 7% only. We are getting more than 7%?

01 July 2015 247 1 View

Are EGFR mutations are dominant or recessive?

Although they are somatic mutations but whether they are dominant or recessive in nature? What is the significance in finding out SNP using Endpoint Genotyping Assay(Roche LC 480 Real Time PCR) or...

24 June 2015 3,819 2 View

How do I determine whether DNA is degraded or not?

Although running the DNA on agarose gel is the best method. Can absorbence be used for determining whether DNA is degraded or not? If yes than what is the criteria?

24 June 2015 1,064 6 View