Yusrifar K Tirta

6 Questions 10 Answers 0 Followers

Questions related from Yusrifar K Tirta

Why my KO mutant shows unexpected band after using CRISPR-Cas9?

Hi, I am doing gene KO in Daphnia using CRISPR-Cas9 by microinjection. I did mutant screening using flanking primer in the DSB site producing 131 bp PCR product. I found one of my lines has two...

03 September 2019 126 4 View

Is there a manual to construct Phylogenetic tree with Kakusan in english?

Hello, I am working with a two genes sequences of octopus (CO1 and NADH1) and want to generate phylogenetic tree from it. I was looking for some suggestion about how to construct the best tree...

03 December 2018 3,965 4 View

Does Plasmodium knowlesi could have a co-infection with other malaria parasites?

Hello, i am researching about Plasmodium knowlesi. i have read this paperhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294981/ The paper still using Pmk8 and Pmkr9 to detect P. knowlesi. But i...

18 February 2016 1,598 5 View

Is there any alternative preservation for kidney tissues besides Bouin fixative for molecular studies?

I'd like to ask a few questions; People said that Bouin fixative is not recommended for molecular studies and ethanol does the work much better. Let say what I am try to fixate is a parasite...

27 November 2015 3,827 3 View

What is the importance of non-human parasite taxonomy studies?

I have been asked a fundamental question about taxonomy studies. He said that it is without question that parasite information is important for medical studies or biotechnology. But what is the...

19 June 2015 4,773 8 View

Which Phylogenetic model should i use to describe the dicyemids ancestor similarity or distance?

I am gonna do ssurRNA sequence alignment of Dicyemid. I want to know the relationship of different dicyemid found in different country. Which phylogenetic model should i use? or if you have some...

31 March 2015 8,787 5 View