How do I determine differences between two hemp seed samples to determine if they are genetically different strains or the same strain?

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Can you use the same sample prep buffer for the GSH/GSSG assay and SOD assay?

I have ordered the SOD colorimetric kit (invitrogen) and a GSH/GSSG ratio assay (abcam, ab205811) and was hoping to use the same brain tissue for both kits. In the methods provided the by the...

11 February 2024 4,390 2 View

How to combine 2 already dichotomized variables into 1 SPSS?

Right now I am wondering what I might be possibly doing wrong with my SPSS syntax. I have got 2 already dichotomized variables and I would need to combine them to make the 3rd one. If var1 and...

20 January 2024 579 10 View

S signal of MS qudrupole (for selected m/z) linear (ms signal (torr) vs concentration) ?

I am using MS quadrupole for on-line detection of diluted gases e. g H2 during TPR H2. How can be MS calibrated for quantitative analysis of hydrogen? When I record signal (torr) for 10 % of H2 in...

15 January 2024 7,149 2 View

Housekeeping genes and a lot of samples?

Hello, I would like to ask a question. I want to do PCR from a large amount of sample, but it does not fit on my plate. This means that in order to test them all, I have to put them on more...

14 January 2024 9,404 1 View

Tools for detecting cross-sample contamination in bacterial short reads?

I have a high number of bacterial cultures I am growing, extracting the DNA from, and sequencing as short reads. Due to the format I'm using (96-well plates), cross-sample contamination is a big...

03 September 2023 4,452 3 View

Isolation cell mRNA with Opentrons OT2?

Hello, I wanted to ask if anyone here works with an Opentrons pipetting station. I would like to experience isolating mRNA from cells, for which we also have a thermoblock and a magnetic block,...

23 February 2023 7,659 1 View

We analyzed an urine sample for chemistry and sediment. We found unidentified objects/artifacts. Do you have any ideas?

We talk about the round oval shape objects. Grid is 15 um. We supossed some kind of drug crystals, but patient has no special medication. Chemistry: blood 2+ leukocytes 2+ pH 7.0 SG 1.009

13 November 2022 112 1 View

How can identify the genes after Trinity assembly (de novo)?

I have files with TRINITY id code and differential expressed values as well as the p values after a de novo transcriptome assemply. What is the proccess after this step to identify which are the...

31 October 2022 8,813 1 View

How sodium tungsten is synthesized?

Hello, is it possible to syntyhesis sodium tungastate (Na2WO4) using ammoium tungstate or tungstic acid.

01 March 2022 5,872 4 View

Which statistical test (tests) should I use for my data?

I am looking at a monoculture diatom biofilm growth on five different materials measured at 6 different time points. So my variables are: dependent variable is "cells per mm^2"; independent...

20 November 2021 3,473 6 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View