How do I design MethyLight primer/probe?

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How to better analyse the Dynamic correlation matrix (DCCM)?

Hi everyone, is there a software or better way to compare and analyze DCCM results between two or more complexes?

28 December 2020 1,821 2 View

How to calculating association and dissociation constants between two protein after MD simulation?

I am trying to calculate both association and dissociation constants between two proteins after MD simulation but my script isn't working. I need someone to kindly assist with script(s) or...

14 December 2020 3,110 1 View

How to run second generation unit root testing on eviews-11?

After running cross section dependence test on Eviews-11, the result shows the presence of cross-section dependence. Possible cause is the interdependence of country considered in the study....

15 November 2020 8,919 1 View

Can I run a panel regression and time series regression for comparison?

I am writing my master dissertation on impact of Microfinance in economic growth and poverty reductio. I have 5 cross-countries trekking 20 years. This makes my data a panel data, however if I run...

01 November 2020 5,650 5 View

In street guide mapping, how can the accuracy be calculated/computed?

Since street guide mapping is a third order job and does not require traverse leg observation like the cadastral, topographic mapping, engineering etc. in which the accuracy can easily be...

15 July 2020 693 1 View

Please i'm a new user of SAS, I need the steps to analyze a soybean data, comparing same dependent variables of 15 locations?

The design is RCBD, 16 entries, 3 reps, 4 blocks, Name. The dependent variables are date of flowering, Flower Color, Date of maturity, Daymat, Grainkha Pop_000, Dayfl, Shat2wk, Shat3wk,...

14 June 2020 571 4 View

How can I adjust a vector map in ArcGIS?

I have an imagery and the road map of the imagery location. I overlaid the imagery on the road map and there is deviation of about 6m. Someone should help me out for the solution.

08 October 2019 1,625 2 View

How do I get detailed explanation of a 4 by 5 Split plot design and how to analyse an experiment with such design using Analysis of Variance (ANOVA)?

Using a 4 by 5 Split Plot Design layout, there are 20 panelists for sensory evaluation. There are 4 different samples at 5 different concentrations. I need detailed explanation on WHOLE PLOT,...

23 October 2018 9,286 3 View

Cutting a dimer insert?

I already dimerized an insert in a vector and now trying to cut the dimer for further cloning; since there are no suitable clean ways to this, I intend to do a full xbal digest of the vector (It...

08 May 2018 5,295 5 View

What do i need to worry about if a restriction enzyme is CpG methylation sensitive? Is there any way to make it work?

I am trying to carryout diagnostic digest with AatII and DraIII (Adei) but the two did not cut as expected. Could it be because of CpG sensitivity? The tests are the paired lanes separated by...

09 October 2017 4,951 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View