How do i calculate the molar extinction coefficient from a absorvance VS wavelegth graph?

10 October 2016 0 9K Report

I dont know my molar concentration and i want to know the Exticion molar coeficcient off my protein in beetween 250 and 800 nm

Badges
Science topic

More Mauro Brás Pereira's questions See All

Which reagent i had to add to a solution of sodium azide to maintain the ionic strength ?

I will increase gradually the concentration of sodium azide , but when it dissociate from sodium i have to maintain the ionic strength to maintain the conditions, because i will need to take...

09 October 2016 9,054 3 View

How do I determinate the concentration of equine heart Myoglobine after a prepared solution where myoglobine was oxidized with ferricyanide ?

So the goal of this experiment it is to determinate the dissociation constant of sodium azide from myoglobine , with different sodium azide concentration by spetroscopic methods. We prepare a...

08 September 2016 1,080 2 View

Why a sample of genomic DNA contaminated with RNA is not good for run on PCR ?

Why all the electrophoresis with RNA are not good DNA PCR RNA comtaminated

04 May 2016 4,355 7 View

How biotine bound to a DNA strand to mark it ?

Description: Biotine; DNA; Biotine marker for DNA

04 May 2016 1,813 1 View

How I explain the difference between the different forms of DNA , A,B,Z,G, and where duplex triplex and quadruplex DNA are related with that ones ?

DNA hoogsteen and watson-crick paring

03 April 2016 3,375 1 View

How does the eletronic transfer mechanism of internal sphere works on inorganic comlexes ?

Block D transition metals

03 April 2016 2,830 5 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

Entropy measure and QSPR modeling in Graph Theor. How to construct the table for lengthy equation?

The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...

30 July 2024 3,126 0 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View