I am doing optogenesis experiment with my cultured neurons. I am kind of confusion how to get exact laser energy expose to my special compartments on my cultured neurons. For example, my 488 laser line total energy is 25 mWatts, and I used 2% to scan the specific part on my cell. How can I calculate the exact laser energy?
Is it a pulsed laser ? If yes, If you know the output power (for example 25 mW) and the repetition rate (10 Hz), there should be about 2.5 mJ per shot,
Otherwise, you can use this (it is very conevient) :
https://gentec-eo.com/?lang=1
Regards
When using a power meter, take care not to overload it! If your laser beam is focused through an objective lens, the power density may be too high and can damage the power meter.
You can either (a) measure the power at the specimen plane without an objective lens, or (b) temporarily reduce the laser power by a known amount by inserting a neutral density filter (e.g. 1:100) into the beam path, and then scale your measurement appropriately.
Check the power meter documentation for the maximum power density specification, and estimate your expected value (for this, you'll need some idea of spot size).
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