How do I calculate amplicon size if I don't know the location of the primer?

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Luigi Marongiu

This looks like a test's question. Anyway, you DO know where the primers are, in fact they are underlined. I counted 62 bases per line, thus you got: (62-5)+62+(62-11). Assuming counting issues due to the fact that the sequence in an image, I would go for the closest to this calculation.

Frederic Lepretre

Hi Maria

primers here are underlined. length of the amplicon will thereby be from 1st base of the upper to the last of the lower. it will additionally be in bp, not in kbp.

stay safe

fred